With the less advanced formats, this is pretty much a no brainer and the quality stored in the mkv should be identical to that on disc. Makemkv, for the most part, just extracts and remuxes the material on disc. But is there anything else like this where the different methods will give different quality? see this here. For instance an Apple TV if you try a 4K MKV with Dolby Atmos it will send PCM - the Shield does not. One such thing I learned is that with Plex, only on the NVIDIA Shield TV Pro will you get lossless audio. I just wanted to know if philosophically/technologically there is any difference at all. But is there anything else like this where the different methods will give different quality? I've tried comparing each, and subjectively I don't notice a major difference, except for perhaps the playback volume being a little different for each device. My question is, is there any difference in audiovisual playback quality between Plex playing an MKV of the UHD disc in this setup, playing the UHD disc itself, or playing an ISO of the disc using Kodi on the NVIDIA Shield TV? Conventional wisdom says no, but it's hard to say.

Projectors don't have Dolby Vision, except for very few (mine does not) so it's really HDR and Dolby Atmos and perhaps a few other parameters, the successful playback of which can depend upon the equipment. I've also wondered about things like HDR and Dolby Vision and advanced audio formats. In the theater, Plex (client) is run off of an NVIDIA Shield TV Pro. With Plex, I rip a Blu-Ray (as well as UHD Blu-Rays) using MakeMKV, and move the MKV onto an NAS, and the Plex server is run off a high-end PC on the network with original playback quality and no rendering.

I really like Plex due to the convenience and portability. The home theater was professionally installed. I have a UHD Blu-Ray player, a 4K Sony projector, a high-end AVR, and top of the line HDMI cables and hardware in between everything. I'm sort of a quality purist with my home theater and I've been trying to figure out if objectively, there is a difference in quality between a few playback methods.

Makemkv 4k rips